Journal: Poultry Science

Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

doi: 10.1016/j.psj.2026.106509

Figure Lengend Snippet: Brefeldin A inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.

Article Snippet: DEF cells were treated with BFA of different concentrations for 36 h, and the cell viability was determined using the cell counting CCK-8 kit (TargetMOI, c0005) according to the manufacturer's instructions.

Techniques: Infection, Control, Fluorescence, Microscopy, Virus, CCK-8 Assay, Viability Assay

Journal: Translational Oncology

Article Title: Construction and validation of golgi apparatus-related genes as predictors of the immune microenvironment and prognosis in colorectal cancer

doi: 10.1016/j.tranon.2026.102714

Figure Lengend Snippet: In Vitro functional experiments of GDI1 . (A). Expression differences of GDI1 among the NCM460, HCT116, and HT29 cell lines; (B)Verification of siRNA efficiency in knocking down GDI1 ; (C) Wound healing assay following GDI1 knockdown; (D) CCK-8 assay after GDI1 knockdown; (E) Apoptosis assay in the HT29 cell line after GDI1 knockdown; (F) Apoptosis assay in the HCT116 cell line after GDI1 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: A suspension of 5000 transfected cells in 100 μL was distributed into each well of a 96-well plate, accompanied by the addition of 10 μL of CCK-8 solution (MCE, HY-K0301).

Techniques: In Vitro, Functional Assay, Expressing, Wound Healing Assay, Knockdown, CCK-8 Assay, Apoptosis Assay